Article,
Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks
Contributors
Teague, K. D.
0000-0001-5454-7405
[1]
[2]
Tellez-Isaias, G.
0000-0002-2416-2747
[1]
[2]
Chai, Jianmin
0000-0001-9341-9932
[1]
[2]
[3]
Petrone-Garcia, V.
0000-0001-6109-6366
[4]
Vuong, C. N.
0000-0001-8663-6816
[1]
[2]
Zhao, Jiangchao
0000-0002-2894-8560
[1]
[2]
Affiliations
- [1] Univ Arkansas, Dept Anim Sci, Div Agr, Fayetteville, AR USA [NORA names: United States; America, North; OECD];
- [2] Univ Arkansas, Dept Anim Sci, Div Agr, Fayetteville, AR USA [NORA names: United States; America, North; OECD];
- [3] Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan, Peoples R China [NORA names: China; Asia, East];
- [4] Univ Nacl Autonoma Mexico, Fac Higher Studies Cuautitlan, Cuautitlan, State of Mexico, Mexico [NORA names: Mexico; America, Central; OECD];
- [5] Hamlet Prot AS, DK-8700 Horsens, Denmark [NORA names: Other Companies; Private Research; Denmark; Europe, EU; Nordic; OECD];
(... more)
Abstract
The objective of this experiment was to investigate the effects of the dietary soy galactooligosac-charides (GOS), raffinose and stachyose, on perfor-mance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch. These 5 treatments were administered to 10 replicate cages of 8 birds. Per-formance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and com-plete blood cell counts were determined at 14 and 21 d. At 21 d, 2 birds per cage were orally gavaged with fluo-rescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage. Additionally, intestinal morphology, crop pre-sumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadrati-cally. Feed conversion ratio increased (P < 0.01) linearly as GOS increased. There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn. There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased. At 14 d, a linear increase (P < 0.05) in blood heterophil to lym-phocyte ratio was observed as dietary GOS increased. Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS. Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococ-cus and Bifidobacterium. Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.
