Article,
Design of a novel multi-layer enzyme membrane reactor for low-fouling, tailored production of oligodextran
Affiliations
- [1] Tech Univ Denmark, Dept Energy Convers & Storage, DK-2800 Lyngby, Denmark [NORA names: DTU Technical University of Denmark; University; Denmark; Europe, EU; Nordic; OECD];
- [2] Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China [NORA names: China; Asia, East];
- [3] Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China [NORA names: China; Asia, East];
- [4] Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China [NORA names: China; Asia, East]
Abstract
Enzymatic conversion processes face challenges in controlling oligosaccharide molecular weight (Mw). Enzymatic membrane reactors (EMRs) with immobilized enzymes address this, but direct enzyme immobilization on the membrane surface can lead to deactivation and reduced hydrolysis efficiency. This study proposes a novel EMR configuration: a three-layer structure. An electrospun porous fibrous layer, modified with PDA, TA, and APTES, serves as a mechanical support layer. A commercial separation membrane is positioned below. This configuration enhances enzyme activity and selectivity. Using a "fouling-induced" technique, immobilized activity of the enzyme (i.e. dextranase) significantly increased to 11.5 mu mol-isomaltose/min, surpassing incubationimmobilized dextranase (0.075 mu mol-isomaltose/min). The additional layer preserves catalytic patterns, reducing fouling and ensuring high selectivity. The EMR configuration excels in producing low Mw oligosaccharides. The catalytic layer achieves 11.3 mu mol-isomaltose/min, while the membrane exhibits exceptional selectivity and stability. The hydrophilic RC10 membrane with small pores performs best. This study highlights the potential of the EMR system for efficient production of stable low Mw oligosaccharides. Insights into optimizing enzyme immobilization strategies and membrane selection benefit enzymatic conversion processes.