Article,
Porcine respiratory coronavirus genome sequences; comparisons and relationships to transmissible gastroenteritis viruses
Contributors
Rasmussen, Thomas Bruun
0000-0002-4241-1559
[2]
Martinenghi, Laura D.
0000-0002-3834-8446
[1]
[2]
Botner, Anette
0000-0002-0558-0222
[1]
Belsham, G. J.
0000-0003-1187-4873
(Corresponding author)
[1]
Affiliations
- [1] Univ Copenhagen, Fac Hlth & Med Sci, Dept Vet & Anim Sci, DK-1870 Frederiksberg, Denmark [NORA names: KU University of Copenhagen; University; Denmark; Europe, EU; Nordic; OECD];
- [2] Statens Serum Inst, Dept Virus & Microbiol Special Diagnost, Copenhagen, Denmark [NORA names: SSI Statens Serum Institut; Governmental Institutions; Denmark; Europe, EU; Nordic; OECD];
- [3] Univ Ghent, Fac Vet Med, Dept Translat Physiol Infectiol & Publ Hlth, B-9820 Merelbeke, Belgium [NORA names: Belgium; Europe, EU; OECD]
Abstract
Porcine respiratory coronavirus (PRCV) was initially detected in Europe, and later in the United States of America (US), in the 1980s. In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences. The whole genome sequences of Danish (1/90 -DK), Italian (PRCV15087/12 III NPTV Parma), and Belgian PRCV (91V44) strains are presented. These sequences were aligned with nine other PRCV sequences from Europe and the US, and 43 TGEV sequences. Following alignment of the PRCV sequences, it was apparent that multiple amino acid variations in the structural proteins were distinct between the European and US strains. The alignments were used to build phylogenetic trees to infer the evolutionary relationships between the strains. In these trees, the European PRCV strains clustered as a separate group, whereas the US strains of PRCV all clustered with TGEVs.
